Card 6
Credit: Sadiya Aysha(160)
Aziz Sir
1. What is nucleotide and nucleoside. Name of the nucleosides
2. Functions of nucleotides, differences between purine and pyrimidine
3. Name of Sugars.
4. Function of nucleotide
5. What is pyrimidine. Raw materials of Pyrimidine
6. Definition of purine nucleus, raw materials of purine synthesis, definition of salvage
7. What is nucleic acid
8. Function of nucleic acid
9. Difference between DNA & RNA
10. Synthesis of histone core. How polynucleosome string is formed?
11. Watson & crick's DNA model
12. Step of DNA Organization - Orders of DNA coiling
Tarana Madam
1. What do you mean by molecular biology and genetics?
2. Name the genetic material.
3. Nitrogen base- Definition types with example.
4. What are the raw materials for purine synthesis?
5. Salvage of nitrogen bases with importance.
6. End products of metabolism of nitrogen bases.
7. Nucleoside- Definition chemistry types.
8. Synthesis of purine and pyrimidine
9. Nucleotide -Definition, chemistry, types, function, figure
10. Nucleic acid -Definition, types, importance.
11. DNA- Definition structure functions, figure types, Watson and Crick model (figure with features)
12. RNA Definition, structure, types, function, figure.
13. Difference between RNA and DNA.
14. Base pairing rule.
15. DNA organization with diagram.
16. Name the building block of DNA.
17. De novo synthesis of purine.
18. Difference between mRNA and tRNA.
19. Difference between purine and pyrimidine.
Aziz Sir
1. Features of Metaphase chromosome
2. Female Male sex chromosome
3. Nucleoprotein definition, function, types. Non-histone protein example
4. Karyotype, Steps of karyotype
5. Difference between nuclear DNA & Mitochondrial DNA
6. Types of Ribosome.
7. What is RNA, Types, Functions
8. What is Centromere, types
9. What is Mitochondrial DNA
Tarana Madam
1. Chromosome- definition, structure, types.
2. Heterochromatin- definition, types.
3. Karyotype- definition with example.
4. Karyotyping- definition and steps.
5. Metaphase chromosome- definition, feature.
6. Short note Nucleoprotein nucleosome.
Aziz Sir
1. Central Dogma
2. Features of replication
3. Steps of replication.
4. Principles of replication
5. Requirement of DNA replication
6. What is Ori?
7. what is replication fork
8. What is DNA sequence. With Type & Example, Function.
9. Okazaki fragment
10. Types & function of DNAP
11. What is ribosome, types, function
12. What is satellite DNA, function;
13. Function of non-coding DNA
Tarana Madam
1. Replication- definition, requirements feature, steps, importance.
2. Name the protein and enzymes required for replication with their functions.
3. What is ori?
4. DNA polymerase- definition, types, function.
5. Define leading Strand and lagging strand
6. Difference between leading and lagging strand
7. DNA provides a template for replication- explain.
8. Short note- Central dogma (VVI), Okazaki fragment, Replication fork, Replication bubble.
Aziz Sir
1. What is Gene?
2. What is Genetic Code?
3. Features of codon ,What is Commaless, Redundancy?
4. What is sense & non sense codon?
5. Name of Non sense codons.
6. Difference between genotype & phenotype,
7. Homozygous, Heterozygous
8. Cell cycle definition, phases, regulator
9. What is G0 phase?
10. What is G1 phase?
11. What is G2 phase?
12. What is P53 & its function, what will happen if it is defective.
Tarana Madam
1. Gene- Definition, structure and function.
2. Definition- Genome, Allele, Trait, Codon, Genotype. Phenotype.
3. What is initiation codon and termination codon?
4. Genetic code- definition, properties.
5. What do you mean by a homozygote?
6. Heterozygotes dominant allele or recessive allele
7. Short note- Cell cycle.
Aziz Sir
1. What is transcription
2. Requirement of transcription
3. Features of transcription
4. Principal of transcription
5. What is RNA polymerase
6. Type of RNA polymerase & Function
7. Transcription unit
8. Composition of promoter sequence
9. Steps of transcription
10. Formation of preinitiation complex
11. Difference between replication & transcription,
12. Post transcriptional modification
13. What is 5' capping & Its action on mRNA
14. RNA editing
15. Splicing: definition, mechanism
Tarana Madam
1. Transcription- definition requirements, features steps.
2. RNA polymerase- definition types functions.
3. Post-transcriptional modification of primary transcript with definition.
4. What do you mean by primary transcript?
5. Difference between DNA polymerase and RNA polymerase?
6. Short note- Reverse transcription
Aziz Sir
1. Definition of translation
2. Requirements of translation
3. Amino acyl-tRNA synthetase
4. Steps of translation
5. Termination process of translation
6. Mechanism of post- translational modification
Tarana Madam
1. Translation- Definition, requirements steps.
2. Name the requirements of initiation of translation in eukaryotes.
3. Post-translational modification with definition.
4. Mention the role of vitamins in post-transcriptional modification.
Aziz Sir
1. Definition & classification of mutation
2. Genetic disorders, definition classification, examples
3. What is frame shift mutation, example
4. pointing mutation and type with example.
5. Estimation of risk in Autosomal Dominant & Recessive Disorders, X linked disorder
6. Name some Autosomal Dominant/Recessive Disorders..
7. Characteristics of Autosomal dominant disorder
Tarana Madam
1. Mutation- Definition, causes, types with example.
2. Point mutation- definition, types, effect.
3. Mutagens- Definition, types.
4. Genetic disorder- Definition, classification, example.
5. Name some autosomal dominant recessive disorder and x-linked dominant recessive disorder
6. What do you mean by x-linked dominant and recessive disorder?
Aziz Sir
1. What is Recombinant DNA
2. Steps of Recombinant DNA
3. PCR, definition, requirements, advantage, disadvantages.
4. Gene therapy: definition, type, characteristics, diseases treated with it
5. Principle of Mandel's law
Tarana Madam
1. Recombinant DNA technology- definition, component, steps and importance.
2. PCR- definition, requirements, steps, advantages importance disadvantages.
3. DNA cloning- Definition, types
4. Biological cloning- Definition, steps.
5. Difference between PCR and biological cloning.
6. Define recombinant DNA.
7. Vector- property and example.
8. DNA fingerprint- definition and its application.
9. Short note Restriction enzyme, RFLP, Genetic polymorphism, Prenatal diagnosis.
Aziz Sir
1. Definition of biotechnology & components
2. Indications for chromosomal analysis
3. Importance of RFLP
4. DNA Finger Print
5. Definition of Genetic Diversity
6. Definition of DNA Library & Types
Tarana Madam
1. Biotechnology- Definition application
2. Application of recombinant DNA technology
3. Gene therapy- definition, strategy, types, steps, importance